ICSI has a high success rate, with fertilisation rates often exceeding 75%.
Egg Fertilisation
At FIV Valencia, we choose the most appropriate treatment for each case: 100% focused on you.
The fertilization of eggs in the Assisted Reproduction laboratory allows us to help achieve pregnancy for those who cannot conceive naturally.
The first step in the IVF process is retrieving mature eggs directly from the patient's ovaries (egg retrieval). At the same time, the partner's sperm is processed in our lab to ensure only the healthiest and most active sperm are used. These sperm are then introduced to the eggs under optimal conditions that mimic the natural environment of the human body.
IVF success rates typically range from 50% to 70%, depending on the quality of both the sperm and eggs. The resulting embryos are monitored and cultured for a few days, and embryologists closely observe their development to select the best embryo for transfer into the uterus.
"To the world, you may be just one person, but to your child, you are the world."
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When is ICSI Recommended?
ICSI is recommended in IVF treatments for the following conditions:
ICSI is primarily recommended for cases of severe male infertility. The decision to proceed with conventional IVF or ICSI is typically made by the embryologist after assessing the semen sample. Whenever possible, FIV Valencia recommends conventional IVF, as it is less invasive and resembles natural fertilization more closely, allowing the egg to select the optimal sperm.
- Low Sperm Count: When the ejaculate contains a significantly low concentration of sperm.
- Poor Sperm Motility: When sperm have difficulty moving, which prevents them from fertilizing the egg.
- Abnormal Sperm Morphology: When a low percentage of sperm have a normal shape, which reduces the likelihood of natural fertilization.
- Low Sperm Count After Semen Processing: When the concentration of sperm post-processing is less than 1 million/ml.
- Restricted Sperm Availability: In cases involving patients who have had vasectomies or those who have undergone radiotherapy or chemotherapy.
- Previous IVF Failure: When conventional IVF has not achieved fertilization.
How Does ICSI Work?
The ICSI procedure includes several key steps:
Ovarian Stimulation
As with conventional IVF, the patient undergoes ovarian stimulation to produce the highest number of mature eggs.
Egg Preparation
Once retrieved, eggs are taken to the embryology lab, where the surrounding granulosa cells are removed to assess their maturity. Only mature eggs are suitable for sperm microinjection.
Sperm Preparation
The sperm sample is processed to obtain the healthiest and most motile sperm, which are then immobilized and prepared for injection.
Microinjection Process
Using a specialised inverted microscope with micro-manipulators, a single sperm is carefully injected into the cytoplasm of each mature egg. The microscope's platform is kept at 37°C, and buffered media are used to prevent pH alterations.
Fertilization Assessment
About 15-18 hours after injection, embryologists examine the eggs under a microscope to check for signs of fertilization. Fertilized eggs, or zygotes, are then cultured in the incubator until they reach the appropriate stage for transfer to the uterus.
Success Rates and Outcomes of IVF-ICSI
This technique is particularly advantageous in cases of severe male infertility, where conventional IVF might not be successful. With ICSI, fertilization does not depend on sperm concentration, motility, or morphology, as only the best sperm are selected for the procedure. Thus, ICSI can achieve success rates similar to conventional IVF with normal sperm samples.
Studies and long-term follow-ups of children born through ICSI have shown no increased risk of genetic abnormalities compared to the general population, confirming the safety and efficacy of this technique. In cases where sperm cannot be obtained through ejaculation, such as with azoospermia, ICSI can be combined with surgical sperm retrieval techniques, like epididymal aspiration or testicular biopsy. Retrieved sperm can be used immediately for ICSI or frozen for future use.
Fertilisation Rate
Fertilisation rate
75 %
Cost of Specialised Laboratory Techniques
While specialised laboratory techniques, such as ICSI, may increase the overall cost of IVF treatment, the potential benefits should be considered. Costs vary depending on the techniques used and the complexity of each case. At FIV Valencia, we offer patients a detailed breakdown of costs during the initial consultation, allowing for an informed decision about
incorporating these advanced methods into the treatment plan. For more information, please refer to our Price List.
Egg Fertilisation
FAQs
How is ICSI different from conventional egg fertilization?
In conventional IVF, sperm are mixed with eggs, allowing for natural fertilization. With ICSI, a single sperm is injected directly into each egg, increasing the chances of fertilization in cases of male infertility or previous IVF failures.
What are the fertilization success rates in IVF?
IVF fertilization success rates typically range from 50% to 70%, depending on factors like egg and sperm quality. ICSI can often achieve a higher fertilization rate, surpassing 75%.
When is ICSI recommended over conventional IVF?
ICSI is recommended for issues with sperm quality (e.g., low count, poor motility, or abnormal morphology) or when previous IVF attempts have failed.
How long does the fertilization process take?
Fertilization usually occurs within 24 hours after combining eggs and sperm. An embryologist examines the eggs under a microscope the next day to confirm successful fertilization
Is fertilization guaranteed with IVF or ICSI?
While IVF and ICSI significantly enhance fertilization chances, there is no absolute guarantee. Success depends on factors such as egg and sperm quality.
Can I choose between conventional fertilization and ICSI?
The decision to use conventional IVF or ICSI is made by the embryologist based on the specifics of your case. Your medical team will discuss the options and provide personalized recommendations.
Does the fertilization method affect the total cost of IVF?
Yes, the fertilization method can impact the cost. ICSI involves additional fees due to the specialised equipment and expertise required.
What happens if fertilization doesn’t occur?
If fertilization does not occur, your medical team will discuss potential reasons and alternative approaches, such as adjustments to protocols or the use of different techniques for future cycles.
Are there risks associated with egg fertilization methods?
Both conventional IVF and ICSI are considered safe, though ICSI involves more egg handling. Your fertility specialist will inform you of any potential risks.
Can fertilized eggs be frozen for future use?
Yes, fertilized eggs (embryos) can be cryopreserved (frozen) for future use, offering the option to use them in subsequent IVF cycles without repeating the entire process.
How is the best sperm selected for ICSI?
During ICSI, the embryologist carefully selects sperm based on appearance, motility, and morphology, increasing the likelihood of successful fertilization.